Cefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic\nhas several side effects and a high degradation rate. For this reason, the preparation and characterization\nof new liposomes that are able to encapsulate this antibiotic seem to be an important research line in the\npharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic\nstructures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium.\nResults showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with\nphosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE).\nThe presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the\nencapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes\nwith phosphatidylcholine was measured. The inhibitory zone in an agar plate for free cefepime was similar\nto that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in\nworking conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All\nobtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can\nbe used in bacterial infections against Escherichia coli with high inhibitory activity.
Loading....